Developing Superhydophobic-Superhydrophilic Surface, Patterning, Biofunctionalization, Miniaturized

Inspiring from ultrasuper hydrophobicity and cleanliness of lotus leaf, we are interested to investigate various surface topography, suitable to generate superhydrophobic surface. On the other hand, there is a possibility to create superhydrophobic-superhydrophilic pattern which will be useful to prepare droplet microarray (DMA). The DMA technique has various advantage towards chemistry as well as biology. It can be used for highthroughput (HT) synthesis of library of compounds in a miniaturized way which will save lot of time and also reduce cost for reactions. The synthesized library can be directly used for HT cell screening towards gene transfection. This will also give us the opportunity to develop novel miniaturized platform for cell based assay. I believe this miniaturized platform will be a promising alternative to microplate technology because they allow for decrease of reagents and cell consumption. In order to achieve next generation widely used technology for HT cell based assay we need to meet following requirements: i) very small reagent and cell consumption; ii) compatibility with low and ultrahigh-throughput; iii) culturing cells in separated compartments to avoid cross- contamination; iv) compatibility with cells of adherent and non-adherent nature; v) parallel and simultaneous addition of chemical libraries as well as other reagents; and vi) easy pipetting-free handling. In best of my knowledge, currently there is no technology which fulfils these requirements, hence there is a clear need of development a new technology which can which can overcome the problem of existing HTS platform. This problem motivated us working in the area of developing new HTS platform and make cell screening affordable and easy to every biological laboratory.